Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies.

@article{Zor1996LinearizationOT,
  title={Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies.},
  author={T Zor and Zvi Selinger},
  journal={Analytical biochemistry},
  year={1996},
  volume={236 2},
  pages={302-8}
}
Determination of microgram quantities of protein in the Bradford Coomassie brilliant blue assay is accomplished by measurement of absorbance at 590 nm. However, as intrinsic nonlinearity compromises the sensitivity and accuracy of this method. It is shown that under standard assay conditions, the ratio of the absorbances, 590 nm over 450 nm, is strictly linear with protein concentration. This simple procedure increases the accuracy and improves the sensitivity of the assay about 10-fold… CONTINUE READING
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