Linear one-step assay for the determination of orthophosphate.

  title={Linear one-step assay for the determination of orthophosphate.},
  author={D A Bencini and James R. Wild and G A O'donovan},
  journal={Analytical biochemistry},
  volume={132 2},
A rapid one-step spectrophotometric assay for orthophosphate that requires a single stable reagent solution is presented. The reagent solution, an aqueous mixture of ammonium molybdate and zinc acetate at pH 5.0, produces a stable complex with orthophosphate that absorbs strongly in the near-visible region of the light spectrum. Response to concentration of phosphate was linear up to 300 microM phosphate with a molar absorptivity of 7200 M-1 cm-1 at 350 nm. The mild conditions for phosphate… CONTINUE READING

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