Linear expression elements: a rapid, in vivo, method to screen for gene functions

@article{Sykes1999LinearEE,
  title={Linear expression elements: a rapid, in vivo, method to screen for gene functions},
  author={Kathryn F Sykes and Stephen A. Johnston},
  journal={Nature Biotechnology},
  year={1999},
  volume={17},
  pages={355-359}
}
The increasing accumulation of genomic sequence information has accentuated the need for new methods to efficiently assess gene function and to prepare reagents to study these functions. Toward solving this general problem in functional genomics, we report a method by which any PCR-amplified open-reading frame (ORF) can be noncovalently linked to a eukaryotic promoter and terminator, and directly injected into animals to produce local gene expression. We also demonstrate that ORFs can be… CONTINUE READING
BETA

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 23 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 14 REFERENCES

Cloning , sequencing , and expression of the apa gene coding for the Mycobacterium tuberculosis 45 / 47kilodalton secreted antigen complex

  • A. Laqueyrerie
  • Infect . Immun .
  • 1995

An improved , heliumdriven biolistic device

  • J. Sanford
  • Technique
  • 1991

Direct transformation of skin and liver tissue in the living mouse

  • R. S. Williams
  • Proc . Natl . Acad . USA
  • 1991

Similar Papers

Loading similar papers…