Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions.

Abstract

The effect of detergent type, pH and temperature during lysis on the DNA elution dose response was studied under non-winding conditions in exponentially growing, plateau-phase and synchronous S-phase CHO cells. Lysis with sodium-N-laurylsarcosine (NLS) increased the DNA elution rate and resulted in higher DNA elution for the same absorbed radiation dose… (More)

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