Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis

@article{Baker2006LineagesOA,
  title={Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis},
  author={Brett J. Baker and Gene W. Tyson and Richard Webb and Judith Flanagan and P. Bernt Hugenholtz and Eric E. Allen and Jillian F. Banfield},
  journal={Science},
  year={2006},
  volume={314},
  pages={1933 - 1935}
}
Novel, low-abundance microbial species can be easily overlooked in standard polymerase chain reaction (PCR)–based surveys. We used community genomic data obtained without PCR or cultivation to reconstruct DNA fragments bearing unusual 16S ribosomal RNA (rRNA) and protein-coding genes from organisms belonging to novel archaeal lineages. The organisms are minor components of all biofilms growing in pH 0.5 to 1.5 solutions within the Richmond Mine, California. Probes specific for 16S rRNA showed… 

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