Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis

@article{Baker2006LineagesOA,
  title={Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis},
  author={B. Baker and G. Tyson and R. Webb and J. Flanagan and P. Hugenholtz and E. Allen and J. Banfield},
  journal={Science},
  year={2006},
  volume={314},
  pages={1933 - 1935}
}
Novel, low-abundance microbial species can be easily overlooked in standard polymerase chain reaction (PCR)–based surveys. We used community genomic data obtained without PCR or cultivation to reconstruct DNA fragments bearing unusual 16S ribosomal RNA (rRNA) and protein-coding genes from organisms belonging to novel archaeal lineages. The organisms are minor components of all biofilms growing in pH 0.5 to 1.5 solutions within the Richmond Mine, California. Probes specific for 16S rRNA showed… Expand
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