Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy.

Abstract

An enhanced sensitivity zero-quantum correlation experiment is proposed for recording (1)H-(13)C correlations of methyl groups in highly deuterated, methyl protonated large proteins. The zero-quantum spectra benefit from TROSY-effects in which both intra- and inter-methyl dipolar relaxation interactions are minimized. Applications to malate synthase G at 5… (More)

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