Limulus hemocyte transglutaminase. Its purification and characterization, and identification of the intracellular substrates.

@article{Tokunaga1993LimulusHT,
  title={Limulus hemocyte transglutaminase. Its purification and characterization, and identification of the intracellular substrates.},
  author={Fuminori Tokunaga and Mitsunori Yamada and Takeshi Miyata and Y L Ding and M Hiranaga-Kawabata and Tatsushi Muta and Sadaaki Iwanaga and Akitada Ichinose and Earl W. Davie},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 1},
  pages={252-61}
}
To investigate further the molecular events of the intracellular coagulation cascade in limulus hemocytes, a transglutaminase (TGase), which may be involved in the formation of a stabilized gel, was purified and characterized. Through the purification procedures consisting of six steps, 1.6 mg of TGase with a specific activity of 940 amine incorporation unit/mg was obtained from 32.4 g of Tachypleus tridentatus hemocytes. The purified TGase gave a single band on SDS-polyacrylamide gel… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

Similar Papers

Loading similar papers…