Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism

  title={Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism},
  author={Azzedine Bounamous and V{\'e}ronique Lehrter and Leila Hadj-Henni and Jean-claude Delecolle and J{\'e}r{\^o}me Depaquit},
  journal={Mem{\'o}rias do Instituto Oswaldo Cruz},
  pages={466 - 472}
A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti, Phlebotomus alexandri, Phlebotomus sergenti, Phlebotomus chabaudi, Phlebotomus riouxi, Phlebotomus perniciosus, Phlebotomus longicuspis, Phlebotomus perfiliewi, Phlebotomus ariasi, Phlebotomus chadlii, Sergentomyia fallax, Sergentomyia minuta, Sergentomyia antennata, Sergentomyia schwetzi, Sergentomyia clydei, Sergentomyia christophersi and Grassomyia… Expand
Exploring the utility of phylogenetic analysis of cytochrome oxidase gene subunit I as a complementary tool to classical taxonomical identification of phlebotomine sand fly species (Diptera, Psychodidae) from southern Europe.
Phlebotomine sand flies (Diptera, Psychodidae) are known to be vectors of several pathogens such as Leishmania and Phlebovirus genera. The identification of phlebotomine sand fly species is currentlyExpand
Direct Multiplex PCR (dmPCR) for the Identification of Six Phlebotomine Sand Fly Species (Diptera: Psychodidae), Including Major Leishmania Vectors of the Mediterranean
A novel, time-saving, cost-effective and easy-to-apply molecular methodology, which avoids sequencing, is designed for the identification of six Eastern Mediterranean sand fly species based on species-specific single-nucleotide polymorphisms of the nuclear 18S rRNA gene. Expand
Species composition, activity patterns and blood meal analysis of sand fly populations (Diptera: Psychodidae) in the metropolitan region of Thessaloniki, an endemic focus of canine leishmaniasis.
August and September are high risk months due to increased sand fly activity levels, animal facilities within or adjacent to urban settlements are highrisk areas and may act as a maintenance and amplification foci for the vector as well as the parasite, and the abundance, ubiquity and feeding behavior of P. perfiliewi and P. simici establishes them as potentially important vectors of Leishmania in the region. Expand
Identification of wild-caught phlebotomine sand flies from Crete and Cyprus using DNA barcoding
Molecular identification of sand flies via DNA barcoding can accurately identify these medically important insects assisting traditional morphological tools, thus helping to assess their implication in Leishmania transmission. Expand
DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
Overall, the molecular data revealed that this PCR-RFLP method does not provide a unique and specific profile for each phlebotomine species tested, suggesting that this method may not be applicable throughout the Mediterranean region as previously proposed. Expand
DNA sequencing confirms PCR-RFLP identification of wild caught Larroussius sand flies from Crete and Cyprus.
Morphology and COI PCR - RFLP were used to identify wild caught Larroussius sand flies belonging to Phlebotomus tobbi, P. perfiliewi, and P. neglectus species from Crete and Cyprus and DNA barcoding will contribute to a molecular identification database to be used for in-depth species studies. Expand
Molecular systematics applied to Phlebotomine sandflies: review and perspectives.
  • J. Depaquit
  • Biology, Medicine
  • Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2014
It is shown that molecular systematics is more frequently used to perform evolutionary systematics than to help in the field of alpha taxonomy, and mitochondrial markers (more than 50% of the markers studied) are preferred to non mitochondrial markers. Expand
Revision of the species composition and distribution of Turkish sand flies using DNA barcodes
The results have epidemiological significance exposing extensive distributions of proven and suspected sand fly vectors in Turkey, including those areas currently regarded as non-endemic for sand fly-borne disease. Expand
Mild-Vectolysis: A Nondestructive DNA Extraction Method for Vouchering Sand Flies and Mosquitoes
Mild-Vectolysis is presented, the first nondestructive DNA extraction methodology for vouchering taxa of mosquitoes and sand flies, which provided sufficient amounts of DNA, tested in a verified barcode (cytochrome oxidase I gene), while preserving their morphological integrity. Expand
Phlebotomine sand flies and Leishmania species in a focus of cutaneous leishmaniasis in Algeria
Cutaneous leishmaniasis is a disease caused by various Leishmania spp., which are transmitted by phlebotomine sand flies. Algeria is one of the most affected countries, with thousands of cutaneousExpand


Towards a rapid molecular identification of the common phlebotomine sand flies in the Mediterranean region.
These methods might represent useful tools for a molecular large scale screening of phlebotomine sand fly species caught in areas where leishmaniasis is endemic, in order to plan appropriate epidemiological surveillance programs for both Leishmania spp. Expand
Mitochondrial DNA Differentiation Between Two Closely Related Species, Phlebotomus (Paraphlebotomus) chabaudi and Phlebotomus (Paraphlebotomus) riouxi (Diptera: Psychodidae), Based on Direct Sequencing and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
A molecular study, based on 40 Algerian and Tunisian specimens, was conducted to distinguish females of these two species, and found that restriction fragment length polymorphism can be applied to identify P. chabaudi and P. riouxi for surveillance purposes. Expand
Sandflies of the Phlebotomus perniciosus complex: mitochondrial introgression and a new sibling species of P. longicuspis in the Moroccan Rif
The discovery of interspecific gene introgression and a new sibling species of P. longicuspis from Morocco mean that previous records of the two morphospecies do not necessarily reflect their true vectorial roles or geographical and ecological distributions. Expand
ITS 2 sequences heterogeneity in Phlebotomus sergenti and Phlebotomus similis (Diptera, Psychodidae): possible consequences in their ability to transmit Leishmania tropica.
The results confirm the validity of the taxa morphologically characterised, and imply the revision of their distribution areas, and seem to be well supported by high degree of variation observed in the present study. Expand
Phylogenetic analysis of Phlebotomus species belonging to the subgenus Larroussius (Diptera, psychodidae) by ITS2 rDNA sequences.
A phylogenetic analysis of the relationships among six representative species of the subgenus Larroussius and one species representatitive of the PhlebotomusSubgenus, assessing sequences of the Second Internal Transcribed Spacer (ITS2) of the ribosomal RNA (rRNA) suggests that P. perniciosus and P. longicuspis are distinct species. Expand
Intraspecific variation within Phlebotomus sergenti Parrot (1917) (Diptera: Psychodidae) based on mtDNA sequences in Islamic Republic of Iran.
There was no consistency between morphotypes and genotypes, but an interrelationship was found between morphometry and morphotypes, and the first combined morphological and molecular studies on P. sergenti s.l. in Iran were performed. Expand
Ecology of leishmaniasis in the South of France. 22. Reliability and representativeness of 12 Phlebotomus ariasi, P. perniciosus and Sergentomyia minuta (Diptera: Psychodidae) sampling stations in Vallespir (eastern French Pyrenees region)
Future research on the dynamics of leishmaniasis outbreaks relative to climate change and agricultural-silvicultural modifications should be very cautiously carried out, while focusing especially on the vector sampling quality and the use of phytoecological maps as vector density indicators. Expand
Intraspecific variability (rDNA ITS and mtDNA Cyt b) of Phlebotomus sergenti in Spain and Morocco.
The aim has been to study the molecular variation on two genes of Spanish populations of P. sergenti, comparing them with Moroccan ones, and the results obtained indicate a high diversity in Spain and the existence in the country of two P.sergenti lineages, a typically Spanish mitochondrial lineage and another one common in Morocco. Expand
Intraspecific Variability of Natural Populations of Phlebotomus sergenti, the Main Vector of Leishmania tropica
In RAPD analysis, distinct clades were formed in accordance with the geographical origin of the specimens, which suggests a gene flow between populations separated spatially by the Amanos mountains, a mountain range of a considerable altitude. Expand
Caractérisation moléculaire et morphologique de deux espèces affines de Paraphlebotomus: Phlebotomus chabaudi Croset, Abonnenc & Rioux, 1970 et P. riouxi Depaquit, Killick-Kendrick & Léger, 1998 (Diptera : Psychodidae)
A molecular study based on 37 Algerian and Tunisian specimens shows the individualisation of two species including an intraspecific variability, and a new character is proposed: the presence of anterolateral teeth of the pharyngeal armature for P. chabaudi. Expand