Limiting dilution analysis of alloreactive T helper cells: precursor frequencies similar to that of alloreactive cytotoxic T cells.


Precursor frequencies for alloreactive T helper cells involved in the generation of primary cytotoxic responses from thymocytes were determined in splenic T cells and selected Lyt-1 lymphocytes by limiting dilution analysis. T helper precursors at frequencies ranging from 1/5000 to 1/13,500 were found in individual experiments in unsensitized selected Lyt-1… (More)


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