Limited proteolysis regulates massive degradation of glycinin, storage 11S globulin from soybean seeds: an in vitro model.


The time course of glycinin hydrolysis by papain was followed using densitometry of SDS-PAGE patterns, quantification of the residual protein and determination of its molecular mass by gel filtration, and by other appropriate methods. The hydrolysis occurs in two steps. In the first step, a limited proteolysis was observed consisting of a gradual detachment… (More)
DOI: 10.1016/j.jplph.2012.06.004


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