Limited proteolysis of a chemically modified third component of human complement, C3, by cathepsin G of human leukocytes.

@article{Matsuda1985LimitedPO,
  title={Limited proteolysis of a chemically modified third component of human complement, C3, by cathepsin G of human leukocytes.},
  author={Takahiko Matsuda and Shigeharu Nagasawa and Takaki Koide and Junko Koyama},
  journal={Journal of biochemistry},
  year={1985},
  volume={98 1},
  pages={
          229-36
        }
}
We have investigated the limited proteolysis of the third component of complement, C3, by a human leukocyte protease, cathepsin G, by using a chemically modified C3, which was prepared by treatment of C3 with methylamine and a fluorescent thiol reagent, N-(dimethylamino-4-methylcoumarinyl)-maleimide (DACM) and was thus named DACM-C3me. Although native C3 was hardly cleaved by cathepsin G, DACM-C3me was cleaved by cathepsin G into three major fragments, which were termed C3c-G (150,000 daltons… CONTINUE READING
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