Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding

  title={Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding},
  author={Jianlong Li and Lori J. Lawson Handley and Lynsey R. Harper and Rein Brys and Hayley Victoria Watson and Bernd H{\"a}nfling},
Environmental DNA (eDNA) metabarcoding is a promising tool for rapid, non-invasive biodiversity monitoring. In this study, eDNA metabarcoding is applied to explore the spatial and temporal distribution of eDNA in two ponds following the introduction and removal of two rare fish species. When two rare species were introduced and kept at a fixed location in the ponds, eDNA concentration (i.e., proportional read counts abundance) of the introduced species typically peaked after two days… 

Read counts from environmental DNA (eDNA) metabarcoding reflect fish abundance and biomass in drained ponds

This research demonstrates that eDNA metabarcoding provides accurate qualitative and quantitative information on fish communities in small ponds, and results are consistent between different methods of DNA capture.

Environmental DNA metabarcoding uncovers environmental correlates of fish communities in spatially heterogeneous freshwater habitats

This study provides further evidence that eDNA metabarcoding is a suitable alternative or complement to conventional fish sampling in reservoirs using a spatiotemporally distributed sampling design covering major environmental gradients.

The true picture of environmental DNA, a case study on harvested fishponds

Significant positive relationships were observed between fish read counts/species site occupancy and fish abundance/biomass with exception of data from pond B in autumn, which supports that eDNA should be applied in optimal conditions.

Monitoring of spatiotemporal occupancy patterns of fish and amphibian species in a lentic aquatic system using environmental DNA

This work demonstrated that a fine‐scale sampling design in combination with eDNA metabarcoding can cover total biodiversity very precisely and allows the construction of consistent spatiotemporal patterns of relative abundance and local distribution of free‐living fish and amphibian species in a lentic ecosystem.

Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water

All three molecular approaches were more sensitive than traditional kick-net sampling for the detection of the quagga mussel in flowing water, and both qPCR and metabarcoding enabled estimates of relative abundance.

Utility of environmental DNA analysis for effective monitoring of invasive fish species in reservoirs

. Dam-mediated biological invasions are a serious problem all over the world. Once established in reservoirs, the invasive species have catastrophic impacts on the river ecosystems downstream, and

Applications of eDNA Metabarcoding for Vertebrate Diversity Studies in Northern Colombian Water Bodies

Environmental DNA metabarcoding is a tool with increasing use worldwide. The uses of such technology have been validated several times for diversity census, invasive species detection, and

Environmental biomonitoring of reef fish community structure with eDNA metabarcoding in the Coral Triangle

Coral reef fishes perform a range of vital ecosystem functions, and can serve as indicators of ecological stress and resilience. However, many species are not observed when using Underwater Visual

Water pre-filtration methods to improve environmental DNA detection by real-time PCR and metabarcoding

Although this study may appear to be a conservative and ancillary experiment, pre-filtration is a simple technique that can not only improve the physical properties of water, such as turbidity, but also the quality of eDNA biomonitoring.

Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story

  • T. BaudryQ. Mauvisseau F. Grandjean
  • Environmental Science
    Proceedings of 1st International Electronic Conference on Biological Diversity, Ecology and Evolution
  • 2021



Factors influencing detection of eDNA from a stream‐dwelling amphibian

The results suggest that eDNA detectability and concentration depend on production rates of individuals, environmental conditions, density of animals, and their residence time.

Spatial Representativeness of Environmental DNA Metabarcoding Signal for Fish Biodiversity Assessment in a Natural Freshwater System

It is confirmed that eDNA metabarcoding is more efficient than a single traditional sampling campaign to detect species presence, especially in rivers, and gives a faithful description of local fish biodiversity in the study system, more specifically within a range of a few kilometers along the river in the authors' study conditions.

Usefulness and limitations of sample pooling for environmental DNA metabarcoding of freshwater fish communities

Because of the decreased detection rates, the pooling strategy is unsuitable for estimating fish species richness, however, this procedure is useful potentially for among-site comparison of representative fish communities.

Transport Distance of Invertebrate Environmental DNA in a Natural River

The results indicate that there may be species' specific transport distances for eDNA and demonstrate for the first time that invertebrate eDNA can persist over relatively large distances in a natural river system.

Next‐generation monitoring of aquatic biodiversity using environmental DNA metabarcoding

It is argued that the proposed DNA‐based approach has the potential to become the next‐generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems.

Environmental DNA metabarcoding of lake fish communities reflects long‐term data from established survey methods

It is demonstrated that eDNA metabarcoding can describe fish communities in large lakes, both qualitatively and quantitatively, and has great potential as a complementary tool to established monitoring methods.

The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding

Encouraging similarity is found in the results obtained from different filtration methods, but the optimal pore size of filter or filter type might strongly depend on the water type under study.

Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake

Comprehensive spatial sampling of England’s largest lake, Windermere, during summer and winter finds broad consistency between results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated to rank abundance determined by long-term data.

The Relationship between the Distribution of Common Carp and Their Environmental DNA in a Small Lake

Results indicate that effective eDNA sampling methods should be informed by fish distribution, as eDNA concentration was shown to vary dramatically between samples taken less than 100 m apart.

Quantification of Environmental DNA (eDNA) Shedding and Decay Rates for Three Marine Fish.

Novel Taqman qPCR assays are designed for three ecologically and economically important marine fish-Engraulis mordax (Northern Anchovy), Sardinops sagax (Pacific Sardine), and Scomber japonicas (Pacific Chub Mackerel) and a simple mass-balance model is derived to estimate fish abundance from eDNA concentration.