Limitations on the use of fused green fluorescent protein to investigate structure-function relationships for the cauliflower mosaic virus movement protein.

@article{Thomas2000LimitationsOT,
  title={Limitations on the use of fused green fluorescent protein to investigate structure-function relationships for the cauliflower mosaic virus movement protein.},
  author={Carole Lesley Thomas and Andrew john Maule},
  journal={The Journal of general virology},
  year={2000},
  volume={81 Pt 7},
  pages={1851-5}
}
To investigate the process of tubule formation for the cauliflower mosaic virus movement protein (CaMV MP), the green fluorescent protein (GFP) was fused to the MP to provide a vital marker for MP location after expression in insect cells. In contrast to the long tubular structures seen previously following baculovirus-based expression of the wild-type MP, the fusion protein produced only aggregates of fluorescing material in the cytoplasm. However, by co-expressing wild-type MP and GFP-MP, or… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 21 extracted citations

The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection.

Proceedings of the National Academy of Sciences of the United States of America • 2008
View 1 Excerpt

Similar Papers

Loading similar papers…