Limitations of HPLC for the detection of β-exotoxin in culture filtrates of Bacillus thuringiensis

Abstract

High performance liquid chromatography (HPLC) was used to detect and quantify β-exotoxin, a phosphorylated adenine nucleotide derivative produced as an excreted metabolite by several strains of Bacillus thuringiensis. The assay was rapid and quantitative for purified β-exotoxin standards. However, peak height failed to correlate β-exotoxin concentration in… (More)
DOI: 10.1007/BF00510421

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