Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds

  title={Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds},
  author={Ming Tien and T. Kent Kirk},
  pages={661 - 663}
The extracellular fluid of ligninolytic cultures of the wood-decomposing basidiomycete Phanerochaete chrysosporium Burds. contains an enzyme that degrades lignin substructure model compounds as well as spruce and birch lignins. It has a molecular size of 42,000 daltons and requires hydrogen peroxide for activity. 
Extracellular ligninase of Phanerochaete chrysosporium Burdsall has no role in the degradation of DDT
The white rot fungus Phanerochaete chrysosporium Burdsall degraded DDT in submerged agitated cultures and the ability of the fungus to metabolize this persistent environmental pollutant is not dependent on the formation of its extracellular lignin-degrading enzyme system. Expand
Crystallization of a Lignin Peroxidase from the White–Rot Fungus Phanerochaete Chrysosporium
The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. TheExpand
Introduction Lignin peroxidases are unusual oxidizing extracellular peroxidases pro­ duced by most of the ligninolytic fungi that cause white rot of wood. 1-4 In the presence of H2O2 they catalyzeExpand
Production of ligninolytic peroxidases by the white rot fungusCoriolopsis occidentalis
SummaryCoriolopsis occidentalis degraded Kraft lignin and dioxan lignin from spruce and, under nitrogen limitation, produced extracellular ligninase, Mn2+-activated peroxidase and veratryl alcohol.Expand
Polymerisation of lignins by ligninases from Phanerochaete chrysosporium
Four major hemoproteins were purified by isoelectric focusing from an extracellular crude enzyme preparation produced by the white rot fungus Phanerochaete chrysosporium under carbon-limited conditions, which resulted in further polymerisation of the lignins and was enhanced by addition of veratryl alcohol to the reaction mixture. Expand
Properties of ligninase from Phanerochaete chrysosporium and their possible applications.
  • M. Tien
  • Chemistry, Medicine
  • Critical reviews in microbiology
  • 1987
The wood-degrading fungus Phanerochaete chrysosporium Burds produces a family of enzymes which degrade lignin and lignin-like substrates. These ligninases exhibit a high degree of homology in beingExpand
Lignin degradeation by white rot fungi
Abstract. The wood-degrading white-rot fungus Phanerochaete chrysosporium, has been the subject of intensive research in recent years and, based upon isolation of the extracellular enzyme ligninase,Expand
Lignocellulose biodegradation and ligninase excretion by mutant strains of phanerochaete chrysosporium hyperproducing cellulases
SummaryLigninolytic activities of mutant strains ofPhanerochaete chrysosporium selected on the basis of cellulase producing criteria were studied using enzymatic and radiorespirometric techniques.Expand
Extracellular ligninolytic enzyme production by Phanerochaete chrysosporium in a new solid-state bioreactor
The production of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) by the fungus Phanerochaete chrysosporium (ATCC 24725) in a new bioreactor, the Immersion Bioreactor, which growsExpand
Lignin-degrading peroxidases from genome of selective ligninolytic fungus Ceriporiopsis subvermispora.
Two new peroxidases oxidize simple and dimeric lignin models and efficiently depolymerize lign in C. subvermispora and suggest the presence of related lignIn-degrading enzymes. Expand


Generation of hydroxyl radical and its involvement in lignin degradation by Phanerochaete chrysosporium.
  • H. Kutsuki, M. Gold
  • Chemistry, Medicine
  • Biochemical and biophysical research communications
  • 1982
Cultures of Phanerochaete chrysosporium produced ethylene from methional and 2-keto-4-thiomethyl butyric acid (KTBA) only under conditions when the organism was competent to degrade [14C]-lignin to 14CO2, indicating that hydroxyl radical plays an important role in lignin degradation by P. chrysOSporium. Expand
Involvement of singlet oxygen in the fungal degradation of lignin.
Results indicate the 1O2 plays an integral role in lignin biodegradation, and also strongly suppressed oxidation of the model compound. Expand
Chemical mechanism of an important cleavage reaction in the fungal degradation of lignin
Abstract Previous research established that lignin substructure model compounds of the non-phenolic β-1 type (R-C α HOH-C β HR′-C γ H 2 OH, in which R,R′ = O-alkyl) are cleaved between C α and C β byExpand
Chromatographic separation of lignin models by molecular weight using sephadex LH-20
Fifteen lignin model compounds with a molecular weight range of 168 to 1076 were examined by descending column chromatography using Sephadex LH-20 with dimethylformamide as solvent. There was a goodExpand
Incorporation of 18O2 and absence of stereospecificity in primary product formation during fungal metabolism of a lignin model compound
Abstract The metabolism of a lignin substructure model compound, 1,2-bis(3-methoxy-4-ethoxyphenyl)propane-1,3-diol (Ia) in ligninolytic cultures of Phanerochaete chrysosporium was studied to helpExpand
Studies on finely divided wood. Part 1. Extraction of lignin with neutral solvents
Previous work on extraction of lignin and cellulose from wood, ground in various ways, and other attempts to isolate 'native lignins' are reviewed. Present opinions about the state of lignin in woodExpand
Synthesis of 14C Labeled 3-Methoxy-4-Hydroxy -α-(2-Methoxy-phenoxy)-β-Hydroxypropiophenone, a Lignin Model Compound
Synthesis of 14 C Labeled 3-Methoxy-4-Hydroxy-�. -(2-Methoxyphenoxy)-�� -Hydroxypropiophenone. Lignin Models a Lignin Model Compound Synthesis Labeled Models Summary Lignin BiodegradationExpand
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins. Expand