LightCycler qPCR optimisation for low copy number target DNA.

  title={LightCycler qPCR optimisation for low copy number target DNA.},
  author={Ian Teo and Jung Woo Choi and John Morlese and Graham Taylor and Sunil Shaunak},
  journal={Journal of immunological methods},
  volume={270 1},
The LightCycler is a rapid air-heated thermal cycler which incorporates a fluorimeter for the detection and quantification of Polymerase Chain Reaction (PCR) amplified products. It provides real-time cycle-by-cycle analysis of product generation. Amplification occurs in glass capillary tubes. The products are detected using a fluorescent double stranded DNA binding dye or fluorescent probes. However, conditions that work well in conventional PCR reactions do not readily translate to the… CONTINUE READING
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Optimizing rapid cycle DNA amplification reactions

  • R. Rasmussen
  • The RapidCyclist. Idaho Technology
  • 1992
Highly Influential
4 Excerpts

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