Light-sheet microscopy using an Airy beam

@article{Vettenburg2014LightsheetMU,
  title={Light-sheet microscopy using an Airy beam},
  author={Tom Vettenburg and Heather I. C. Dalgarno and Jonathan Nylk and Clara Coll-Lladó and David E. K. Ferrier and Tom{\'a}{\vs} {\vC}i{\vz}m{\'a}r and Frank Gunn-Moore and Kishan Dholakia},
  journal={Nature Methods},
  year={2014},
  volume={11},
  pages={541-544}
}
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all… 

Airy Beams for Light-sheet Microscopy

A fundamental limitation of LSM with Gaussian beam illumination is the rapid divergence of the beam which prevents a uniformly thin light-sheet, thus impeding high-resolution imaging over a large

Planar Airy beam light-sheet for two-photon microscopy

TLDR
It is shown how a planar light-sheet can be formed from the curved propagation-invariant Airy beam, and the resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field ofview without the need for deconvolution.

Optimization of Airy beam profile to improve light-sheet microscopy illumination

Light-sheet microscopy (LSM) is an imaging technique that has the facilities of fast and high contrast 3D imaging with minimal light exposure. In LSM, a conventional Gaussian beam has a rapid

Fast Fluorescence Microscopy with Light Sheets

TLDR
This review explains why light sheet microscopes are much faster and gentler than other established fluorescence microscopy techniques and introduces new volumetric imaging schemes and highlights of selected biological applications.

Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy

Light sheet fluorescence microscopy enables high-resolution imaging of thick biological samples. By restricting the fluorescence excitation to a single plane, rapid wide-field image acquisition is

Light-Sheet Fluorescence Microscopy With Structured Light

A compact Airy beam light sheet microscope with a tilted cylindrical lens.

TLDR
This work presents a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light- Sheet microscope OpenSPIM, to achieve Airy light-sheets illumination, which brings wide field single-photon light- sheet imaging to a broader range of endusers.

Integrated single- and two-photon light sheet microscopy using accelerating beams

TLDR
This work demonstrates the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes, and opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Airy light-sheet Raman imaging.

TLDR
It is demonstrated that a digitally scanned Airy beam increases the Raman imaging throughput rates by more than an order of magnitude than conventional diffraction-limited beams.
...

References

SHOWING 1-10 OF 29 REFERENCES

Wavefront corrected light sheet microscopy in turbid media

Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations.

Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM).

Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in

Multiview light-sheet microscope for rapid in toto imaging

TLDR
The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which is demonstrated through in toto imaging of the embryonic development of Drosophila melanogaster.

High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy

We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal

Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles

TLDR
It is found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.

Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination

Resolution enhancement in a light-sheet-based microscope (SPIM).

TLDR
SPIM outperforms 2hnu-FM and FM, while CFM has a better axial resolution at NAs above 0.8, and the discrepancies are found to be less than 5%.

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

TLDR
Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.

3D adaptive optics in a light sheet microscope.

TLDR
A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish, showing improvements in image quality whilst recording a 3D "z-stack" and showing how the aberrations come from varying depths in the fish.

Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media.

TLDR
The beam's propagation stability along one straight line enables the use of a confocal line principle, resulting in a significant increase in image contrast, while the axial resolution could be improved by nearly 100% relative to the standard light-sheet techniques using scanned Gaussian beams.