Light-sheet fluorescence microscopy for quantitative biology

  title={Light-sheet fluorescence microscopy for quantitative biology},
  author={Ernst H. K. Stelzer},
  journal={Nature Methods},
are degraded much like fluorophores3 and thus are unavailable for vital metabolic processes. Second, the number of fluorophores in any volume element at any given time is finite, and fluorophores can degrade upon excitation. As a consequence, the number of photons that can be retrieved from a fluorophore-labeled specimen is limited. Finally, life on Earth is adapted to the solar flux, which is less than 1.4 kW/m2. This might not be a hard limit, but it indicates that irradiance should not… CONTINUE READING
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Theory and Practice of Scanning Optical Microscopy

  • T. Wilson, C.J.R. Sheppard
  • 1984

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