Light scatter as an adjunct to cellular immunofluorescence in flow cytometric systems.

@article{Hansen1982LightSA,
  title={Light scatter as an adjunct to cellular immunofluorescence in flow cytometric systems.},
  author={W. Peter Hansen and R A Hoffman and Stephen H. Ip and Kathleen W. Healey},
  journal={Journal of clinical immunology},
  year={1982},
  volume={2 3 Suppl},
  pages={32S-41S}
}
Optical flow cytometry has provided a rapid method for the analysis of cell surface markers to accompany the recent proliferation of monoclonal antibodies specific to subsets of peripheral blood cells. This review presents the physical principles by which cell populations are distinguished in flow cytometry, thereby comparing the complementary roles of light scatter from the interior structure of cells, fluorescence from labeled antibody, and optical interactions with enzyme labels. The role of… CONTINUE READING

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