Light-regulated tetracycline binding to the Tet repressor.

  title={Light-regulated tetracycline binding to the Tet repressor.},
  author={Jayoung Moon and Jongsik Gam and Seung Goo Lee and Young-Ger Suh and Jeeyeon Lee},
  volume={20 9},
Elucidation of the signal-transmission pathways between distant sites within proteins is of great importance in medical and bioengineering sciences. The use of optical methods to redesign protein functions is emerging as a general approach for the control of biological systems with high spatiotemporal precision. Here we report the detailed thermodynamic and kinetic characterization of novel chimeric light-regulated Tet repressor (TetR) switches in which light modulates the TetR function. Light… CONTINUE READING