Light microscopy: an ongoing contemporary revolution

  title={Light microscopy: an ongoing contemporary revolution},
  author={Siegfried Weisenburger and Vahid Sandoghdar},
  journal={Contemporary Physics},
  pages={123 - 143}
The optical microscope is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe’s nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving subwavelength structures were not feasible. The Nobel Prize in 2014 for super-resolution fluorescence microscopy marks a clear recognition that the old beliefs have to be revisited. In this article, we present a… 

Optical nanoscopy with contact microlenses overcomes the diffraction limit

The fundamental nature of the diffraction limit means that it forms an unavoidable barrier in any far-field imaging system. In addition, Abbe, Rayleigh, Sparrow, and Houston resolution criteria show

Polarization-Encoded Colocalization Microscopy at Cryogenic Temperatures

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision

Interferometric scattering (iSCAT) microscopy with optimized reference wave

The understanding of nanoscale biological processes is limited by the level of details we can achieve when observing their dynamics. Addressing molecules of interest using fluorescent labels is the

Insights on reflection: new ideas gained from comparing femtosecond laser development, microscopy, and patterning

The development of various forms of super-resolution microscopy have paralleled the resolution gains in lithography. From micron limited resolution 30 years ago, to nm resolution today. In biology,

Spotlight on microspherical nanoscopy: Experimental quantification of super-resolution

It is argued that the super-resolution capability of contact microspheres stems from an image magnification effect taking place in close proximity to the object with contributions of its optical near-fields.

Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers

In recent years, optical super-resolution by microspheres and microfibers emerged as a new paradigm in nanoscale label-free and fluorescence imaging. However, the mechanisms of such imaging are still

Super‐resolution microscopy by movable thin‐films with embedded microspheres: Resolution analysis

Microsphere‐assisted imaging has emerged as an extraordinary simple technique of obtaining optical super‐resolution. This work addresses two central problems in developing this technology: i)

Simultaneous dual-color imaging on singel-molecule level on a widefield microscope and applications

ed from [1–4] The epifluorescence microscope, as we know it today, has come a long way. Starting as a spin-off to the UV microscope at the beginning of the twentieth century, its development over the

Label-free nanoscopy with contact microlenses: Super-resolution mechanisms and limitations

Despite all the success with developing super-resolution imaging techniques, the Abbe limit poses a severe fundamental restriction on the resolution of far-field imaging systems based on diffraction

Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

The recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed, and both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space.



Far-Field Optical Nanoscopy

Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.

Resolution enhancement techniques in microscopy

AbstractWe survey the history of resolution enhancement techniques in microscopy and their impact on current research in biomedicine. Often these techniques are labeled superresolution, or enhanced

Light and video microscopy

Breaking the diffraction barrier in fluorescence microscopy by optical shelving.

The breaking of the diffraction resolution barrier in far-field fluorescence microscopy is reported by transiently shelving the fluorophore in a metastable dark state by confining the fluorescence emission to a spot whose diameter is a fraction of the wavelength of light.

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy

3D stochastic optical reconstruction microscopy (STORM) is demonstrated by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy, allowing the 3D morphology of nanoscopic cellular structures to be resolved.

Optical microscopy using a single-molecule light source

This work presents optical images taken with a single molecule as a point-like source of illumination, by combining fluorescence excitation spectroscopy with shear-force microscopy, which has potential for achieving molecular resolution in optical microscopy.

Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution.

  • M. Gustafsson
  • Physics
    Proceedings of the National Academy of Sciences of the United States of America
  • 2005
Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.

Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination

Saturated patterned excitation microscopy--a concept for optical resolution improvement.

The theory of nonlinear patterned excitation microscopy is developed for achieving a substantial improvement in resolution by deliberate saturation of the fluorophore excited state and the effects of photon noise are included in the simulations.

Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution

A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.