Light-induced dark states of organic fluochromes enable 30 nm resolution imaging in standard media.

@article{Baddeley2009LightinducedDS,
  title={Light-induced dark states of organic fluochromes enable 30 nm resolution imaging in standard media.},
  author={David Baddeley and Isuru D Jayasinghe and Christoph Cremer and Mark B. Cannell and Christian Soeller},
  journal={Biophysical journal},
  year={2009},
  volume={96 2},
  pages={
          L22-4
        }
}
We show that high quantum efficiency fluorophores can exhibit reversible photobleaching. This observation provides the basis for an imaging technique we call reversible photobleaching microscopy. We demonstrate applicability of this technique using antibody labeled biological samples in standard aqueous (or glycerol based) media to produce far-field images at approximately 30 nm resolution. Our novel method relies on intense illumination to reversibly induce a very long-lived (>10 s) dark state… CONTINUE READING

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