Light‐Wavelength‐Based Quantitative Control of Dihydrofolate Reductase Activity by Using a Photochromic Isostere of an Inhibitor

@article{Mashita2019LightWavelengthBasedQC,
  title={Light‐Wavelength‐Based Quantitative Control of Dihydrofolate Reductase Activity by Using a Photochromic Isostere of an Inhibitor},
  author={Takato Mashita and Toshiyuki Kowada and Hiroto Takahashi and Toshitaka Matsui and Shin Mizukami},
  journal={ChemBioChem},
  year={2019},
  volume={20}
}
Photopharmacology has attracted research attention as a new tool for achieving optical control of biomolecules, following the methods of caged compounds and optogenetics. We have developed an efficient photopharmacological inhibitor—azoMTX—for Escherichia coli dihydrofolate reductase (eDHFR) by replacing some atoms of the original ligand, methotrexate, to achieve photoisomerization properties. This fine molecular design enabled quick structural conversion between the active “bent” Z isomer of… Expand
4 Citations
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