Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.

@article{Chu1988LigationOO,
  title={Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.},
  author={Byron C. H. Chu and Leslie E. Orgel},
  journal={Nucleic acids research},
  year={1988},
  volume={16 9},
  pages={
          3671-91
        }
}
We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide by a phosphoramidate bond. The adducts are mixed and reduced with dithiothreitol. The dithiothreitol is then removed by dialysis. Oxidation by atmospheric oxygen occurs to yield… CONTINUE READING