Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks.

@article{Steigerwald1990LigationmediatedPI,
  title={Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks.},
  author={S D Steigerwald and Gerd. P. Pfeifer and Arthur D. Riggs},
  journal={Nucleic acids research},
  year={1990},
  volume={18 6},
  pages={1435-9}
}
DNA methylation at specific sites is most frequently studied by use of methylation-sensitive restriction endonucleases and Southern blotting. We report here that the sensitivity of this method can be increased several-hundred-fold by applying a ligation-mediated polymerase chain reaction (LM-PCR) procedure following enzyme treatment. DNA is cleaved simultaneously with two restriction enzymes, one sensitive and one insensitive to methylation. After cleavage, a gene-specific oligonucleotide… CONTINUE READING
Highly Cited
This paper has 28 citations. REVIEW CITATIONS