Ligation-free ribosome profiling of cell type-specific translation in the brain


Ribosome profiling has emerged as a powerful tool for genome-wide measurements of translation, but library construction requires multiple ligation steps and remains cumbersome relative to more conventional deep-sequencing experiments. We report a new, ligation-free approach to ribosome profiling that does not require ligation. Library construction for ligation-free ribosome profiling can be completed in one day with as little as 1 ng of purified RNA footprints. We apply ligation-free ribosome profiling to mouse brain tissue to identify new patterns of cell type-specific translation and test its ability to identify translational targets of mTOR signaling in the brain.

DOI: 10.1186/s13059-016-1005-1

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@inproceedings{Hornstein2016LigationfreeRP, title={Ligation-free ribosome profiling of cell type-specific translation in the brain}, author={Nicholas Hornstein and Daniela Torres and Sohani Das Sharma and Guomei Tang and Peter Canoll and Peter A Sims}, booktitle={Genome Biology}, year={2016} }