Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase.

Abstract

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here how T4 DNA ligase can be used for efficient construction of DNA molecules having one 5' end but two distinct 3' ends that extend from the 2' and 3' carbons… (More)

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@article{MendelHartvig2004LigasemediatedCO, title={Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase.}, author={Maritha Mendel-Hartvig and Anil Kumar and Ulf Landegren}, journal={Nucleic acids research}, year={2004}, volume={32 1}, pages={e2} }