Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles

@article{Erster2007LigandIS,
  title={Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles},
  author={Oran Erster and Miriam Eisenstein and Mordechai Liscovitch},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={393-395}
}
The ligand interaction scan (LIScan) method is a general procedure for engineering small molecule ligand–regulated forms of a protein that is complementary to other 'reverse' genetic and chemical-genetic methods for drug-target validation. It involves insertional mutagenesis by a chemical-genetic 'switch', comprising a genetically encoded peptide module that binds with high affinity to a small-molecule ligand. We demonstrated the method with TEM-1 β-lactamase, using a tetracysteine hexapeptide… CONTINUE READING