Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography.

  title={Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography.},
  author={Myriam M. Altamirano and Adrian Woolfson and Alena Donda and Abdijapar Shamshiev and Luis Brise{\~n}o-Roa and Nicholas W Foster and Dmitry B. Veprintsev and Gennaro De Libero and A. R. Fersht and C{\'e}sar Milstein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={98 6},
CD1 is an MHC class I-like antigen-presenting molecule consisting of a heavy chain and beta(2)-microglobulin light chain. The in vitro refolding of synthetic MHC class I molecules has always required the presence of ligand. We report here the use of a folding method using an immobilized chaperone fragment, a protein disulphide isomerase, and a peptidyl-prolyl cis-trans isomerase (oxidative refolding chromatography) for the fast and efficient assembly of ligand-free and ligand-associated CD1a… CONTINUE READING


Publications referenced by this paper.

CD 1 : From Structure to Function

  • F. Calabi, C. Yung Yu, C. A. G. Bilsland, C. Milstein
  • Immunogenetics of the Major Histocompatibility…
  • 1991

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