Ligand immobilization using thiol - disulphide exchange

  • Published 2002


This Application Note describes an immobilization procedure based on thioldisulphide exchange, providing a valuable complement to the established amine coupling reaction. Thiol coupling is particularly useful for acidic ligands. The method is exemplified by immobilization of acidic peptides from the interleukin-2 receptor, the acidic carcinoembryonic antigen (CEA) and antibodies against β2-microglobulin. The last-named example provides a direct comparison of the thiol and amine coupling methods. Abstract

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@inproceedings{2002LigandIU, title={Ligand immobilization using thiol - disulphide exchange}, author={}, year={2002} }