Ligand-driven vectorial folding of ribosome-bound human CFTR NBD1.

@article{Khushoo2011LiganddrivenVF,
  title={Ligand-driven vectorial folding of ribosome-bound human CFTR NBD1.},
  author={Amardeep Khushoo and Zhongying Yang and Arthur E. Johnson and William R. Skach},
  journal={Molecular cell},
  year={2011},
  volume={41 6},
  pages={682-92}
}
The mechanism by which protein folding is coupled to biosynthesis is a critical, but poorly understood, aspect of protein conformational diseases. Here we use fluorescence resonance energy transfer (FRET) to characterize tertiary structural transitions of nascent polypeptides and show that the first nucleotide-binding domain (NBD1) of human CFTR, whose folding is defective in cystic fibrosis, folds via a cotranslational multistep pathway as it is synthesized on the ribosome. Folding begins… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 67 references

Cotranslational structure acquisition of nascent polypeptides monitored by NMR spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America • 2010
View 6 Excerpts
Highly Influenced

Protein folding on the ribosome.

Current opinion in structural biology • 2010
View 5 Excerpts
Highly Influenced

Probing ribosome-nascent chain complexes produced in vivo by NMR spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America • 2009
View 4 Excerpts
Highly Influenced

Phenylalanine-508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function.

Proceedings of the National Academy of Sciences of the United States of America • 2008
View 4 Excerpts
Highly Influenced

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