• DOI:10.1074/JBC.270.50.29698
Corpus ID: 19275865

Ligand-directed Immunoaffinity Purification and Properties of the One-carbon, Reduced Folate Transporter

@article{Chiao1995LiganddirectedIP,
  title={Ligand-directed Immunoaffinity Purification and Properties of the One-carbon, Reduced Folate Transporter},
  author={J. Chiao and C. Yang and K. Roy and J. Pain and F. Sirotnak},
  journal={The Journal of Biological Chemistry},
  year={1995},
  volume={270},
  pages={29698 - 29704}
}

• J. Chiao, C. Yang, +2 authors F. Sirotnak
• Published 1995
• Biology, Medicine
• The Journal of Biological Chemistry

Almost complete purification (>95%) of the 46-kDa murine, one-carbon, reduced folate transporter (RFT) at a recovery of 20% was obtained by ligand-directed immunoaffinity fractionation from transporter overproducing L1210/R83 cells. These cells were labeled with the N-hydroxysuccinimide ester of [3H]aminopterin (AMT), the isolated plasma membrane alkaline washed to remove nonintegral membrane proteins, detergent-solubilized, and RFT-separated on an anti-AMT antibody-protein G-Sepharose column… Expand

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