Ligand-directed Immunoaffinity Purification and Properties of the One-carbon, Reduced Folate Transporter

  title={Ligand-directed Immunoaffinity Purification and Properties of the One-carbon, Reduced Folate Transporter},
  author={J. Chiao and C. Yang and K. Roy and J. Pain and F. Sirotnak},
  journal={The Journal of Biological Chemistry},
  pages={29698 - 29704}
Almost complete purification (>95%) of the 46-kDa murine, one-carbon, reduced folate transporter (RFT) at a recovery of 20% was obtained by ligand-directed immunoaffinity fractionation from transporter overproducing L1210/R83 cells. These cells were labeled with the N-hydroxysuccinimide ester of [3H]aminopterin (AMT), the isolated plasma membrane alkaline washed to remove nonintegral membrane proteins, detergent-solubilized, and RFT-separated on an anti-AMT antibody-protein G-Sepharose column… Expand
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