Ligand-dependent folding of the three-way junction in the purine riboswitch.

  title={Ligand-dependent folding of the three-way junction in the purine riboswitch.},
  author={Colby D Stoddard and Sunny D. Gilbert and Robert T Batey},
  volume={14 4},
Riboswitches are highly structured cis-acting elements located in the 5'-untranslated region of messenger RNAs that directly bind small molecule metabolites to regulate gene expression. Structural and biochemical studies have revealed riboswitches experience significant ligand-dependent conformational changes that are coupled to regulation. To monitor the coupling of ligand binding and RNA folding within the aptamer domain of the purine riboswitch, we have chemically probed the RNA with N… CONTINUE READING