Ligand-dependent equilibrium fluctuations of single calmodulin molecules.

@article{Junker2009LiganddependentEF,
  title={Ligand-dependent equilibrium fluctuations of single calmodulin molecules.},
  author={Jan Philipp Junker and Fabian Ziegler and Matthias Rief},
  journal={Science},
  year={2009},
  volume={323 5914},
  pages={633-7}
}
Single-molecule force spectroscopy allows superb mechanical control of protein conformation. We used a custom-built low-drift atomic force microscope to observe mechanically induced conformational equilibrium fluctuations of single molecules of the eukaryotic calcium-dependent signal transducer calmodulin (CaM). From this data, the ligand dependence of the full energy landscape can be reconstructed. We find that calcium ions affect the folding kinetics of the individual CaM domains, whereas… CONTINUE READING

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