Ligand binding and functional characterization of muscarinic acetylcholine receptors on the TE671/RD human cell line.

@article{Bencherif1991LigandBA,
  title={Ligand binding and functional characterization of muscarinic acetylcholine receptors on the TE671/RD human cell line.},
  author={Merouane Bencherif and Ronald J. Lukas},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1991},
  volume={257 3},
  pages={946-53}
}
Cells of the TE671/RD human clonal line express a finite number (Bmax) of about 350 fmol/mg of membrane protein) of apparently noninteracting, high-affinity binding sites (KD of 0.07 nM and a Hill coefficient close to unity, nH = 0.94) for the muscarinic acetylcholine receptor (mAChR) radio antagonist, tritium-labeled quinuclidinyl benzilate [( 3H]QNB). The rank order potency of selective antagonists that inhibit specific [3H]QNB binding is: atropine greater than 4-DAMP (4-diphenylacetoxy-N… CONTINUE READING