Ligand binding‐dependent functions of the lipocalin NLaz: an in vivo study in Drosophila

@article{Ruiz2014LigandBF,
  title={Ligand binding‐dependent functions of the lipocalin NLaz: an in vivo study in Drosophila},
  author={Mario Ruiz and Maria D Ganfornina and Colin E. Correnti and Roland K. Strong and Diego Sanchez},
  journal={The FASEB Journal},
  year={2014},
  volume={28},
  pages={1555 - 1567}
}
Lipocalins are small extracellular proteins mostly described as lipid carriers. The Drosophila lipocalin NLaz (neural Lazarillo) modulates the IIS pathway and regulates longevity, stress resistance, and behavior. Here, we test whether a native hydrophobic pocket structure is required for NLaz to perform its functions. We use a point mutation altering the binding pocket (NLazL130R) and control mutations outside NLaz binding pocket. Tryptophan fluorescence titration reveals that NLazL130R loses… 
View on Wiley
digital.csic.es
13 Citations
Background Citations
1
Methods Citations
2

13 Citations

Citation Type

Citation Type

Identification of a novel tetrameric structure for human apolipoprotein-D
TLDR
Investigation of the native structure of apolipoprotein-D derived from plasma, breast cyst fluid (BCF) and cerebrospinal fluid shows that it predominantly forms a ∽95 to ∽100 kDa tetramer, which indicates Apolipopprotein-D rarely exists as a free monomer under physiological conditions and provides insights into novel native structures of APD and into oligomerisation behaviour in the lipocalin family.
Identification of a novel tetrameric structure for human apolipoprotein-D.
Protecting cells by protecting their vulnerable lysosomes: Identification of a new mechanism for preserving lysosomal functional integrity upon oxidative stress
TLDR
A previously unknown biological function of ApoD, member of the finely regulated and evolutionary conserved gene family of extracellular Lipocalins, is uncovered, set a lipoprotein-mediated regulation of lysosomal membrane integrity as a new mechanism at the hub of many cellular functions, critical for the outcome of a wide variety of neurodegenerative diseases.
Lipocalins in Arthropod Chemical Communication
TLDR
Large numbers of lipocalin genes in the same species, their wide divergence, both within and between species, and their expression in chemosensory organs suggest that such expansion may have occurred under environmental pressure, supporting the hypothesis that lipocalins may be involved in chemical communication in arthropods.
BKCa (Slo) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster
TLDR
The presence of BKCa channels in mitochondria of Drosophila is established and its novel physiological role in regulating mitochondrial structural and functional integrity, and lifespan is ascertained.
Apolipoprotein D-mediated preservation of lysosomal function promotes cell survival and delays motor impairment in Niemann-Pick type A disease
Odorant-binding proteins of mammals.
  • P. Pelosi, W. Knoll
  • Biology, Chemistry
    Biological reviews of the Cambridge Philosophical Society
  • 2021
TLDR
It is concluded that OBPs of mammals are specifically dedicated to pheromonal communication, and those of insects, which are completely different in sequence and structure, include carriers for general odorants in addition to those dedicated topheromones.
PROTEINS OF THE INTEGUMENTARY SYSTEM OF THE HONEYBEE, Apis mellifera.
TLDR
Gene ontology functional analysis revealed that the higher proportions of the identified proteins have molecular functions related to catalytic and structural molecule activities, are involved in metabolic biological processes, and pertain to the protein class of structural or cytoskeletal proteins and hydrolases.
Basic mechanisms of longevity: A case study of Drosophila pro-longevity genes
Extracellular Vesicles Secreted by Astroglial Cells Transport Apolipoprotein D to Neurons and Mediate Neuronal Survival Upon Oxidative Stress
TLDR
It is demonstrated that a classical neuroprotective protein, Apolipoprotein D (ApoD), expressed by glial cells and known to promote functional integrity and survival of neurons, is exclusively transported by EVs from astrocytes to neurons, where it gets internalized.
...
1
2
...

References

SHOWING 1-10 OF 80 REFERENCES
Lipid‐binding properties of human ApoD and Lazarillo‐related lipocalins: functional implications for cell differentiation
TLDR
The results show that ApoD is necessary and sufficient to allow for RA differentiating activity, and both human and Drosophila NLaz successfully deliver RA to immature neurons, driving neurite outgrowth.
Molecular interactions of the neuronal GPI‐anchored lipocalin Lazarillo
TLDR
It is demonstrated that Lazarillo oligomerizes in solution and can form clusters in the plasma membrane when expressed and GPI‐anchored to the cell surface, however it is unable to mediate cell–cell adhesion.
Grasshopper Lazarillo, a GPI-anchored Lipocalin, increases Drosophila longevity and stress resistance, and functionally replaces its secreted homolog NLaz.
Comparative ligand-binding analysis of ten human lipocalins.
Control of Metabolic Homeostasis by Stress Signaling Is Mediated by the Lipocalin NLaz
TLDR
The results show that JNK-NLaz signaling antagonizes IIS and is critical for metabolic adaptation of the organism to environmental challenges, suggesting that similar interactions represent an evolutionarily conserved system for the control of metabolic homeostasis.
Sex-dependent modulation of longevity by two Drosophila homologues of human Apolipoprotein D, GLaz and NLaz
The plant Apolipoprotein D ortholog protects Arabidopsis against oxidative stress
TLDR
This study provides the first functional evidence that a plant lipocalin is involved in modulating tolerance to oxidative stress, and is in agreement with recently published data showing that overexpression of ApoD enhances tolerance to antioxidant stress and increases life span in mice and Drosophila.
Hydrophobic ligand binding properties of the human lipocalin apolipoprotein M Published, JLR Papers in Press, May 24, 2007.
TLDR
The binding of retinol and retinoic acid supports the hypothesis that apoM is a lipocalin, and the physiological relevance of this binding has yet to be elucidated.
Galline Ex-FABP is an antibacterial siderocalin and a lysophosphatidic acid sensor functioning through dual ligand specificities.
Anticalins small engineered binding proteins based on the lipocalin scaffold.
...
1
2
3
4
5
...