Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila.

@article{Romeijn2005Lig4AR,
  title={Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila.},
  author={Ron J. Romeijn and Marcin M. Gorski and Martijn A van Schie and Jasprina N. Noordermeer and L. H. Mullenders and Wouter Ferro and Albert Pastink},
  journal={Genetics},
  year={2005},
  volume={169 2},
  pages={795-806}
}
Site-specific double-strand breaks (DSBs) were generated in the white gene located on the X chromosome of Drosophila by excision of the w(hd) P-element. To investigate the role of nonhomologous end joining (NHEJ) and homologous recombination (HR) in the repair of these breaks, the w(hd) P-element was mobilized in flies carrying mutant alleles of either lig4 or rad54. The survival of both lig4- and rad54-deficient males was reduced to 25% in comparison to the wild type, indicating that both NHEJ… CONTINUE READING