Life after the synthetic cell

@article{Bedau2010LifeAT,
  title={Life after the synthetic cell},
  author={Mark A. Bedau and George M. Church and Steen Rasmussen and Arthur L. Caplan and Steven A. Benner and Martin Fussenegger and Jim Collins and David Deamer},
  journal={Nature},
  year={2010},
  volume={465},
  pages={422-424}
}
Nature asked eight synthetic-biology experts about the implications for science and society of the “synthetic cell” made by the J. Craig Venter Institute (JCVI). The institute's team assembled, modified and implanted a synthesized genome into a DNA-free bacterial shell to make a self-replicating Mycoplasma mycoides. 

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References

Total synthesis and cloning of a gene coding for the ribonuclease S protein.

TLDR
The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified, facilitating tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.