Manual for maintenance of multi-host ixodid ticks in the laboratory
The life cycle of Amblyomma romitii Tonelli-Rondelli, 1939 is reported for the first time, using rabbits (Oryctolagus cuniculus) for larvae and capybara (Hydrochoerus hydrochaeris) for nymphs and adults, as experimental hosts. Developmental periods of free-living stages were observed in an incubator at 27 ± 1°C, 80 ± 10% relative humidity (RH), and 24-h darkness. The life cycle of A. romitii in the laboratory could be completed in an average period of 216.4 d. The overall sex ratio (M:F) was 1:1.4. The results showed that rabbits are quite suitable as experimental hosts for the larval stages of A. romitii, while capybaras are suitable experimental hosts for nymphs and adults.