Library-based methods for identification of soluble expression constructs.

@article{Yumerefendi2011LibrarybasedMF,
  title={Library-based methods for identification of soluble expression constructs.},
  author={Hayretin Yumerefendi and Danielle C Desravines and Darren J Hart},
  journal={Methods},
  year={2011},
  volume={55 1},
  pages={38-43}
}
When expression or crystallisation of a protein target in its wild-type full-length form proves problematic, a common strategy is to divide it into subconstructs comprising one or more domains. Rational construct design is not always successful, especially with targets for which there are few similar sequences to generate multiple sequence alignments. Even when this is possible, expression constructs may still fail to yield soluble protein, commonly expressing insolubly or at unusable yields… CONTINUE READING

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