Liberation and osmotic properties of the protoplasts of Micrococcus lysodeikticus and Sarcina lutea.

Stable protoplasts may be released from MiCrococcua Zysodeikticus and Sarcina Zutea by digestion of the cell-wall with lysozyme in sucrose or NaCl solutions having an osmotic pressure of some 25 atmospheres, but not in glycerol solutions of the same osmotic pressure. The stability of the protoplasts depends not only upon the depression of the water activity… CONTINUE READING