• Corpus ID: 248722094

Leveraging isotopologues as a general strategy to image neurotransmitters with vibrational microscopy

@inproceedings{Dorlhiac2022LeveragingIA,
  title={Leveraging isotopologues as a general strategy to image neurotransmitters with vibrational microscopy},
  author={Gabriel F. Dorlhiac and Markita P. Landry and Aaron M. Streets},
  year={2022}
}
Chemical neurotransmission is central to neurotypical brain function but also implicated in a variety of psychiatric and neurodegenerative diseases. The release dynamics of neurotransmitters is correlated with but distinct from electrical signal propagation. It is therefore necessary to track neurotransmitter modulation separately from electrical activity. Here, we present a new approach for imaging deuterated neurotransmitter molecules with vibrational microscopy in the cell-silent window… 

Figures from this paper

References

SHOWING 1-10 OF 77 REFERENCES
Label-free imaging of neurotransmitters in live brain tissue by multi-photon ultraviolet microscopy
TLDR
This review shows that MP ultraviolet (MP-UV) microscopy is an effective but underutilized method for imaging monoamine neurotransmitters in neurones and brain tissue.
Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors
TLDR
The development and validation of dLight1 is reported, a novel suite of intensity-based genetically encoded dopamine indicators that enables ultrafast optical recording of neuronal dopamine dynamics in behaving mice and permits robust detection of physiologically and behaviorally relevant dopamine transients.
Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum
TLDR
Fluorescent false neurotransmitter 200 (FFN200) was developed, a vesicular monoamine transporter 2 (VMAT2) substrate that selectively traces monoamine exocytosis in both neuronal cell culture and brain tissue, and represents the first report suggestive of presynaptically silent neuromodulatory synapses.
Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor
TLDR
NIRCats can be used to measure electrically and optogenetically evoked dopamine release in brain tissue, revealing hotspots with a median size of 2 µm and it is demonstrated that nIRCats are compatible with dopamine pharmacology and show D2 autoreceptor modulation of evokes dopamine release.
Label-free dopamine imaging in live rat brain slices.
TLDR
The technique opens up a previously inaccessible mid-ultraviolet spectral regime for label-free imaging of native molecules in live tissue and shows that individual dopamine vesicles/vesicular clusters can be imaged in cultured rat brain slices, thereby providing a direct visualization of the intracellular events preceding dopamine release induced by depolarization or amphetamine exposure.
Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential.
TLDR
A vibrational spectroscopic signature of neuronal membrane potentials identified through hyperspectral stimulated Raman scattering (SRS) imaging of patched primary neurons is reported and is interpreted as ion interactions on the CH3 Fermi resonance peak in proteins.
Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain
TLDR
A pH-responsive FFN probe, FFN102, which as a polar dopamine transporter substrate selectively labels dopamine cell bodies and dendrites in ventral midbrain and dopaminergic synaptic terminals in dorsal striatum, demonstrates that, by bringing together organic chemistry and neuroscience, molecular entities can be generated that match the endogenous transmitters in selectivity and distribution.
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo.
TLDR
The general procedure to design, realize, and test any type of CNiFER is described, given that any GPCR can be used to create a novel CNiFer and that there are approximately 800 GPCRs in the human genome.
A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice
TLDR
The new isoBeRST-Halo hybrid indicators achieve single-trial voltage imaging of membrane potential dynamics from cultured hippocampal neurons or cortical neurons in brain slices and provide an important complement to voltage imaging in neurobiology.
...
...