Leukotoxicity of pyoverdin, production of reactive oxygen species, and effect of UV radiation.

@article{Becerra2001LeukotoxicityOP,
  title={Leukotoxicity of pyoverdin, production of reactive oxygen species, and effect of UV radiation.},
  author={Cecilia Becerra and In{\'e}s Albesa and Alberto Jorge Eraso},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={285 2},
  pages={414-8}
}
Pyoverdin was purified by solvent extraction, gel filtration, and ionic exchange chromatography. Assays of cytotoxic of pyoverdin were done with human leukocytes and macrophages from the peritoneum of mice. Both cell quantities showed a significant reduction. Death was followed by lysis in a dose-dependent form. The mechanism of action of pyoverdin involved the stimulation of reactive oxygen species (ROS) measured by Nitroblue Tetrazolium (NBT) reaction and chemiluminescence (CL). UV radiation… CONTINUE READING