Leukocytospermia and sperm preparation - a flow cytometric study

@article{Ricci2009LeukocytospermiaAS,
  title={Leukocytospermia and sperm preparation - a flow cytometric study},
  author={Giuseppe Ricci and Sandra Perticarari and Rita Boscolo and Roberto Simeone and Monica Martinelli and Leo Fischer-Tamaro and Secondo Guaschino and Gianni Presani},
  journal={Reproductive Biology and Endocrinology : RB\&E},
  year={2009},
  volume={7},
  pages={128 - 128}
}
BackgroundLeukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i) to compare the efficiency of swim-up and density-gradient centrifugation techniques in removing… 
The influence of leukocytospermia on the outcomes of assisted reproductive technology
TLDR
The results indicate that the leukocytospermia may not have a negative effect on the outcomes of ICSI or IMSI cycles, but it seems that it is necessary to more precisely determine the effects, if any, of seminal leukocytes on fertilisation and implantation processes.
Biomarker-Based Flow Cytometric Semen Analysis for Male Infertility Diagnostics and Clinical Decision Making in ART
TLDR
This chapter reviews the emerging biomarker-based andrological approaches to automated semen analysis by high-throughput flow cytometry (FC) and discusses approaches in the context of clinical decision making and pregnancy outcomes in ART couples, particularly with regard to the possibility of single embryo transfer.
Role of asymptomatic genital tract inflammation in normozoospermic infertile men undergoing intracytoplasmic sperm injection
TLDR
Estimation of seminal elastase is more accurate in detecting asymptomatic male genital tract inflammation compared with peroxidase stain, however, routine determination of seminalElastase in asymPTomatic normozoospermic infertile men undergoing ICSI is of limited value.
Centrifugation stress reduces the responsiveness of spermatozoa to a capacitation stimulus in in vitro‐aged semen
TLDR
Density gradient centrifugation alters the physiological property of spermatozoa for controlled capacitation, which may influence the success rates of centrifuged semen in assisted reproductive technologies and confound interpretation of capacitationAssessment revealed a highly conserved ability of in vitro‐aged spermatoza to specifically respond to bicarbonate.
Human β-defensin 1 in follicular fluid and semen: impact on fertility
TLDR
The results possibly suggest that hBD-1 could influence oocyte and sperm quality, and could improve, when exogenously added, sperm motility.
DHR123: an alternative probe for assessment of ROS in human spermatozoa
TLDR
DHR123 may be considered a suitable probe for estimating oxidants produced by human spermatozoa, and can present heterogeneity in oxidant production between different samples.
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
TLDR
The differences in protein levels found on comparison of DC with SU spermatozoa indicate possible dissimilarities in their glycolytic metabolism and DNA methylation and suggest that DC cells may have a better capacitation potential.
Cryopreservation of Low Number of Human Spermatozoa; Which is Better: Vapor Phase or Direct Submerging in Liquid Nitrogen?
TLDR
Cryopreservation of a small number of spermatozoa with the DS method showed superior outcomes with regard to motility, viability and chromatin configuration.
Ultrastructural Morphology of Sperm from Human Globozoospermia
TLDR
This is the first demonstration of the existence of relevant morphological homologies between the tail coiling in animal model and human globozoospermia, a rare disorder characterized by the presence of sperm with round head, lacking acrosome.
...
...

References

SHOWING 1-10 OF 47 REFERENCES
Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function.
TLDR
The carryover of leukocytes into the washed sperm preparations profoundly influenced the fertilizing potential of the spermatozoa via mechanisms that were associated with the production of reactive oxygen species.
Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity
TLDR
While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation.
Leukocyte detection in human semen using flow cytometry.
TLDR
Flow cytometry using monoclonal antibodies was seen to be a simple, reproducible method that enables leukocytes in semen to be accurately detected and to identify WBC subpopulations without preliminary purification procedures.
A new multiparameter flow cytometric method for human semen analysis.
TLDR
The combination of Syto 16/7-AAD provides a sensitive assay to detect sperm apoptosis and the multiparameter flow cytometric method described offers the possibility of a simultaneous, simple, rapid and accurate assessment of several semen parameters.
Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes.
TLDR
Sperm apoptosis did not seem to be correlated with semen quality and in the absence of genito-urinary infection, one of the main functions of seminal leukocytes is probably to provide for the removal of apoptotic sperm.
Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma.
TLDR
Low concentrations of leukocytes are a common feature of the human ejaculate and can impair sperm function, particularly in the absence of seminal plasma, which has implications for the understanding of the importance ofLeukocytospermia in defining the fertility of human spermatozoa in vivo and in vitro.
Is leukocytospermia clinically relevant?
Indirect immunofluorescence using monoclonal antibodies for the detection of leukocytospermia: comparison with peroxidase staining
TLDR
Determination of peroxidase‐positive cells to detect leukocytospermia, the standard procedure recommended by the WHO, was compared with the indirect immunofluorescence technique using monoclonal antibodies, indicating that the standard method of detection of seminal leukocytes may be inaccurate.
...
...