• DOI:10.1016/S0167-4781(02)00298-1
Corpus ID: 39160371

Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley.

@article{Cormack2002LeucineZW,
  title={Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley.},
  author={R. S. Cormack and T. Eulgem and P. Rushton and Petra K{\"o}chner and K. Hahlbrock and I. Somssich},
  journal={Biochimica et biophysica acta},
  year={2002},
  volume={1576 1-2},
  pages={
          92-100
        }
}

R. S. Cormack, T. Eulgem, +3 authors I. Somssich

Published 2002

Biology, Medicine

Biochimica et biophysica acta

Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one-hybrid system. In yeast, both proteins interacted sequence-specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo-binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene… CONTINUE READING

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