• Biology, Medicine
• Published in Biochimica et biophysica acta 2002
• DOI:10.1016/S0167-4781(02)00298-1

Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley.

@article{Cormack2002LeucineZW,
  title={Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley.},
  author={R S Cormack and Thomas Eulgem and Paul J. Rushton and Petra K{\"o}chner and K. -H. Hahlbrock and Imre E Somssich},
  journal={Biochimica et biophysica acta},
  year={2002},
  volume={1576 1-2},
  pages={
          92-100
        }
}

• R S Cormack, Thomas Eulgem, +3 authors Imre E Somssich

Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one-hybrid system. In yeast, both proteins interacted sequence-specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo-binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene… CONTINUE READING

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