Lethal mutations in the structural gene of an outer membrane protein (OmpA) of Escherichia coli K12

@article{Freudl1985LethalMI,
  title={Lethal mutations in the structural gene of an outer membrane protein (OmpA) of Escherichia coli K12},
  author={Roland Freudl and Gabi Braun and Ingrid Hindennach and U. Henning},
  journal={Molecular and General Genetics MGG},
  year={1985},
  volume={201},
  pages={76-81}
}
The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revealed that in one mutant type the last CO2H-terminal residue… CONTINUE READING