Lessons learned from microsatellite development for nonmodel organisms using 454 pyrosequencing

@article{Schoebel2013LessonsLF,
  title={Lessons learned from microsatellite development for nonmodel organisms using 454 pyrosequencing},
  author={C. N. Schoebel and S. Brodbeck and D. Buehler and C. Cornejo and J. Gajurel and H. Hartikainen and D. Keller and M. Leys and {\vS}. Ř{\'i}{\vc}anov{\'a} and G. Segelbacher and S. Werth and D. Csencsics},
  journal={Journal of Evolutionary Biology},
  year={2013},
  volume={26}
}
Microsatellites, also known as simple sequence repeats (SSRs), are among the most commonly used marker types in evolutionary and ecological studies. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing is a straightforward approach to develop a high number of microsatellite markers. Therefore, developing microsatellites using 454 pyrosequencing has become the method of choice for marker… Expand
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