Lessons from human teratomas to guide development of safe stem cell therapies

@article{Cunningham2012LessonsFH,
  title={Lessons from human teratomas to guide development of safe stem cell therapies},
  author={Justine J. Cunningham and Thomas M. Ulbright and Martin F. Pera and Leendert H. J. Looijenga},
  journal={Nature Biotechnology},
  year={2012},
  volume={30},
  pages={849-857}
}
The potential for the formation of teratomas or other neoplasms is a major safety roadblock to clinical application of pluripotent stem cell therapies. Preclinical assessment of the risk of tumor formation in this context poses considerable scientific and regulatory challenges, especially because animal xenograft models may not properly reflect the long-term tumorigenic potential of human cells. A better understanding of the biology of spontaneously occurring teratomas and related tumors in… 

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  • Thomas F. Peter W. Yishai Ivana Nissim Christoph Jennifer Allison Andrews Avior Barbaric Benvenisty Bock BreT. Allison S. Yamanaka
  • Biology
    Nature Communications
  • 2018
EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm or endoderm lineages, are sufficient to assess the differentiation potential of PSCs, and only the teratoma assay offers evidence of malignant potential.
...

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