Lessons from Nature: microRNA-based shRNA libraries

  title={Lessons from Nature: microRNA-based shRNA libraries},
  author={Kenneth Chang and Stephen J Elledge and Gregory J Hannon},
  journal={Nature Methods},
Loss-of-function genetics has proven essential for interrogating the functions of genes and for probing their roles within the complex circuitry of biological pathways. In many systems, technologies allowing the use of such approaches were lacking before the discovery of RNA interference (RNAi). We have constructed first-generation short hairpin RNA (shRNA) libraries modeled after precursor microRNAs (miRNAs) and second-generation libraries modeled after primary miRNA transcripts (the Hannon… CONTINUE READING
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