Lesion (in)tolerance reveals insights into DNA replication fidelity.

  title={Lesion (in)tolerance reveals insights into DNA replication fidelity.},
  author={Eva Freisinger and Arthur P. Grollman and Holly Miller and Caroline Kisker},
  journal={The EMBO journal},
  volume={23 7},
The initial encounter of an unrepaired DNA lesion is likely to be with a replicative DNA polymerase, and the outcome of this event determines whether an error-prone or error-free damage avoidance pathway is taken. To understand the atomic details of this critical encounter, we have determined the crystal structures of the pol alpha family RB69 DNA polymerase with DNA containing the two most prevalent, spontaneously generated premutagenic lesions, an abasic site and 2'-deoxy-7,8-dihydro-8… CONTINUE READING